Budapest Biostruct Course on

Basics in Crystallographic Data Collection and Data Processing

2015 DECEMBER 14-18, BUDAPEST, HUNGARY


The course is supported within the Biostruct-X consortium


Under Patronage of Professor Dr Ferenc Faigl,

Dean of the Faculty of Chemical Engineering and Bioengineering
 

 

 

Programme

 

 

Budapest Biostruct Course on

Basics in Crystallographic Data Collection and Data Processing

 

within the framework of the Biostruct-X project.

Dates: December 14th  2015 – December 18th 2015

Venue: Budapest University of Technology and Economics Biostruct Laboratory

 

 

 

Day 0 Monday 14th December

 

Arrival

2000 Lecturers’ Dinner (1118 Budapest, Budaörsi út 20a)  (this is a preliminary date, we will arrange the start  so that everyone can join who arrives on Monday or Sunday)

 

 

Day 1 Tuesday 15th December

 

Lectures at the Ch building Room A20

 

8:30 –                   Registration

9:00 –  9:15         Welcome address and Opening remarks

                             Ferenc Faigl, dean of the Faculty of Chemical and BioEngineering, Budapest University of Technology and Economics,

                             Beata Vertessy (organiser)

9:15 – 10:00        Lecture 1 From molecules to their structure: roadmap of structure solution in crystallography  Fred Dyda                                          10:00 – 10:45      Lecture 2  State of the art methods of to macromolecular crystallography Fred Dyda

 

10:45 – 11:15      Coffee break

 

11:15 – 12:00      Lecture 3 Symmetry and data collection strategies Sándor Brockhauser

 

12:15 – 14:00      Lunch

 

14:00 – 14:45      Lecture 4 Introductory lecture on data processing, integration and scaling Karina Sparta

14:45 – 15:30      Lecture 5 Introduction to iMosflm Harry Powell

 

15:30 – 16:00      Coffee break

 

16:00 – 17:00      Practical sessions, handling protein crystals and data collection (in two groups, rotation) Zoltan Gal, Ibolya Leveles

16:00 – 16:30

                              Group  A:  Data collection practical in the Biostruct lab 

                              Group B :  Handling protein crystals for data collection (Ch building Room A20)

16:30 – 17:00

                              Group A: Handling protein crystals for data collection (Ch building Room A20)

                              Group B: Data collection practical in the Biostruct lab 

17:00 – 17:50      Group A &B Visit of the protein crystallography laboratory, Eötvös Loránd University

 

Sightseeing in the city

 

 

Day 2 Wednesday 16th December

8:30                   Meeting at Building CH, walk to Building R

9:00-12:20  Computer lab practicals, Building R IV floor

9:00 – 10:30       Data processing with iMosflm Harry Powell

 

10:30 – 10:50      Coffee break

 

10:50 – 12:20      Data processing with XDS and XDSapp Karine Sparta

(optimally these two practicals could concentrate on the particular strength of the respective software, somehow reducing redundancy)

 

12:20 – 13:50      Lunch

 

13:50-18:00  Lecture session at Ch building A20

13:50 – 14:40      Lecture 6 Phase determination methods-I (SAD, MAD density modifications, SHELXD, Sharp ) Fred Dyda

14:40 – 15:30      Lecture 7 Macromolecular phasing using HKL2MAP and SHELX Thomas Schneider

 

15:30 – 15:50      Coffee break

 

15:50 – 16:40      Lecture 8 Structure determination using CCP4 Andrey Lebedev

16:40 – 17:30      Lecture 9 Structure determination using Phaser Gabor Bunkoczi

17:30 – 18:15      Lecture 10 Model building and refinement Orsolya Barabás, Fred Dyda

 Q&A

 

 

Day 3 Thursday 17th December

 

9:00-18:00  Computer lab practicals, Building R IV floor

9:00 – 10:30       Experimental phasing using HKL2MAP and SHELX Thomas Schneider

 

10:30 – 10:50      Coffee break

 

10:50 – 12:20      Molecular replacement in CCP4 Andrey Lebedev

 

12:20 – 13:50       Lunch

 

13:50 – 14:20       Lecture 11  The BESSY structural biology beamlines Karine Sparta

14:20 – 14:50       Lecture  12  The EMBL structural biology beamlines at PETRA III Thomas Schneider

 

14:50 – 15:05      Coffee break

 

15:05 – 16:35      Molecular replacement by Phaser Gabor Bunkoczi

 

16:20 – 16:50      Coffee break

 

16:50 – 18:20      Model building and refinement (Coot, PDB-redo, Molprobity) Veronika Harmat, Orsolya Barabas

 Q&A

19:30 –                 Course Dinner, Hotel Mediterran (1118 Budapest, Budaörsi út 20a)  

 

 

Day 4 Friday 18th December

 

Lectures at Ch building A20

9:00 – 9:45         Lecture 13  X-ray data collection at home source and synchrotron Zoltán Gál

9:45 – 10:30       Lecture 14 Tips and tricks for a synchrothron visit Sándor Brockhauser

 

10:30 – 10:50     Coffee break

 

10:50 – 11:35     Lecture 15 Model validation Veronika Harmat

11:35 – 12:20     Lecture 16 How to publish data 

 

12:20 – 12:40     Closing remarks Beata Vertessy

 

Departure